Additional file 2: of Guide for library design and bias correction for large-scale transcriptome studies using highly multiplexed RNAseq methods

Layout of reference samples and the qualities. There are seven quality measures provided in the STRTprep pipeline; (i) TOTAL_READS is a read count after the redundant read exclusion based on UMI; (ii) REDUNDANCY is redundancy based on UMI; (iii) MAPPED_RATE is the rate of mapped reads; (iv) SPIKEIN_5END_RATE is the 5′-end capture rate of spike-in molecules; (v) CODING_5END_RATE is the 5′-end capture rate of protein coding genes; (vi) CODING_READS is reads that were aligned to exons of protein coding genes; and (vii) CODING_RATE is calculated by CODING_READS/(TOTAL_READS*MAPPED_RATE). See also detailed instruction at https://github.com/shka/STRTprep/blob/v3dev/doc/result.md#outbygenesamples_allcsv . (TXT 16 kb)