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Transcriptome and translatome of killing supernatant cultured human monocytes

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE195524
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To investigate whether metoprolol treatment could affect the transcriptome and translatome of killing supernatant cultured human monocytes PBMC were isolated from buffy coat of healthy blood donors by Ficoll gradients, and CD14+ monocytes were isolated from PBMC with anti-CD14 magnetic beads (Miltenyi Biotec). CD14+ monocytes were cultured with killing supernatant for 5 days and treated with metoprolol or PBS prophylactically or conditionally and then were sent for both ribo-seq and RNA-seq
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2023-07-31
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