RPA XRT screening of oligos for potato mop-top virus (PMTV) detection using RNA from a tuber.

Sample onset time and intensity are provided as averages for two replicates of each combination. Sample onset time was determined by evaluating change in fluorescence intensity over time, set arbitrarily at 60 RFU/min. Intensity is an evaluation of the fluorescence output of a given oligo combination. Combinations that failed to amplify PMTV resulted in a “-” for no onset and intensities below 200 mV.