Promotion of parallel DNA quadruplexes by a yeast telomere binding protein: a circular dichroism study.
收藏PubMed Central1994-08-02 更新2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC44461/
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资源简介：
Repressor-activator protein 1 (RAP1) has an essential role in the maintenance of yeast telomeres. Yeast telomeric DNA consists of simple repeated G-rich sequences that are bound by RAP1. We have found that RAP1, in addition to its known binding activity for double-stranded DNA, interacts with the G-rich strand containing guanine base (G)-tetrads. We show here using circular dichroism spectroscopy that RAP1 promotes the formation of one particular type of DNA quadruplex, parallel G4-DNA. Furthermore, RAP1 is able to bind to both preformed parallel and antiparallel DNA quadruplexes. These results have implications for the possible use of DNA quadruplexes in telomere-telomere association in vivo. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1994-08-02
