Tracking total bacterial abundances and Alcanivorax specific abundances with qPCR in Pensacola Beach, June 2010 to June 2011

Bacterial abundances were tracked approximately every 6 weeks for one year beginning in June 2010 in Pensacola Beach. Sand samples were visibly assessed as oil contaminated or "clean" and total genomic DNA was extracted using a MoBio powersoil DNA extraction kit. Total bacterial 16S rRNA gene abundances were quantified using taqman qPCR with 331F/772R primers and a 515R probe, while Alcanivorax 16S rRNA gene abundances were quantified with the same probe with 464F/675R primer. This dataset supports the publication: Kostka, J.E., Prakash, O., Overholt, W.A., Green, S.J., Freyer, G., Canion, A., Delgardio, J., Norton, N., Hazen, T.C., & Huettel, M. (2011). Hydrocarbon-Degrading Bacteria and the Bacterial Community Response in Gulf of Mexico Beach Sands Impacted by the Deepwater Horizon Oil Spill. Applied and Environmental Microbiology, 77(22): 7962-7974.本数据集于2010年6月起在彭萨科拉海滩（Pensacola Beach）开展为期一年的监测工作，每约6周记录一次细菌丰度。砂样本通过目视评估被划分为石油污染组与"清洁"组，总基因组DNA采用MoBio PowerSoil DNA提取试剂盒完成提取。总细菌16S rRNA基因丰度通过TaqMan定量聚合酶链式反应（qPCR）进行定量，所用引物为331F/772R、探针为515R；食烷菌属（Alcanivorax）16S rRNA基因丰度则采用相同探针，搭配464F/675R引物完成定量。本数据集支持以下已发表学术论文：Kostka, J.E.、Prakash, O.、Overholt, W.A.、Green, S.J.、Freyer, G.、Canion, A.、Delgardio, J.、Norton, N.、Hazen, T.C. 与 Huettel, M.（2011）。《深水地平线漏油事件影响下墨西哥湾海滩砂中的烃降解细菌及细菌群落响应》，刊载于《应用与环境微生物学》（Applied and Environmental Microbiology），第77卷第22期，页码范围7962-7974。