Small RNA-Seq analysis in various Prunus species infected with ALSV vector. Prunus

We assessed the VIGS efficiency of ALSV vectors in a wide range of Prunus species and cultivars, and found that ALSV vectors could successfully induce VIGS of endogenous PDS genes in several cultivars of apricot, sweet cherry, and almond. Small RNA-Seq analysis revealed preferential accumulation of antisense siRNAs in PDS-silenced leaves, suggesting their role as guide RNAs in VIGS.