Cold-water coral microbiomes (Lophelia pertusa) from Gulf of Mexico and Atlantic Ocean: raw data

The files in this data release are the raw deoxyribonucleic acid (DNA) sequence files referenced in the submitted journal article by Christina A. Kellogg, Dawn B. Goldsmith and Michael A. Gray entitled "Biogeographic comparison of Lophelia-associated bacterial communities in the western Atlantic reveals conserved core microbiome". They represent a 16S ribosomal ribonucleic acid (rRNA) gene amplicon survey of the coral’s microbiomes completed using Roche 454 pyrosequencing with Titanium series reagents. Samples from the Gulf of Mexico were collected in 2009 and 2010. Samples from the Atlantic Ocean were collected in 2009. The raw data files associated with this study have also been submitted to the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) under Bioproject number PRJNA305617. Minimum information about a marker gene (MIMARKS) compliant metadata is provided in "Lophelia metadata", which is included in the data download file. For more information, please contact Christina Kellogg at the U.S. Geological Survey (USGS) St. Petersburg Coastal and Marine Science Center, 600 4th Street South, St. Petersburg, Florida, USA, 33701; Telephone: [phone]; email: [e-mail].本数据集发布所包含的文件为Christina A. Kellogg、Dawn B. Goldsmith与Michael A. Gray合著的已投稿期刊论文中引用的原始脱氧核糖核酸（deoxyribonucleic acid, DNA）序列文件，该论文标题为《西大西洋与石珊瑚（Lophelia）共生的细菌群落生物地理比较揭示保守核心微生物组》。本数据集为该珊瑚微生物组的16S核糖体核糖核酸（ribosomal ribonucleic acid, rRNA）基因扩增子测序调查成果，采用罗氏454焦磷酸测序技术配合Titanium系列试剂完成。墨西哥湾样本采集于2009年与2010年，大西洋样本采集于2009年。本研究相关的原始数据文件已提交至美国国家生物技术信息中心（National Center for Biotechnology Information, NCBI）的序列读取档案（Sequence Read Archive, SRA），生物项目编号为PRJNA305617。符合标记基因最小信息（Minimum Information about a Marker Gene, MIMARKS）标准的元数据已包含在数据下载包内的"Lophelia元数据"文件中。如需更多信息，请联系美国地质调查局（U.S. Geological Survey, USGS）圣彼得堡海岸与海洋科学中心的Christina Kellogg，地址：美国佛罗里达州圣彼得堡市南第四街600号，33701；电话：[phone]；邮箱：[e-mail]。