Transcriptional response of wild type and dmr6 mutants of tomato to Xanthomonas gardneri. Transcriptional response of wild type and dmr6 mutants of tomato to Xanthomonas gardneri

This study evaluates the transcriptional response of Solanum lycopersicum cv. Fla. 8000 (wild-type and dmr6 mutants) to the pathogen Xanthomonas gardneri 153. Overall design: Wild type and mutant (Sldmr6-1.1 and Sldmr6-1.2) plants were grown on soil (Miracle-Gro Supersoil Potting Soil) in a growth chamber at 25°C under a 16h light/8h dark photoperiod. Four individual plants of each genotype (wild type, Sldmr6-1.1 and Sldmr6-1.2 mutants) were used for RNA sequencing. Two leaves from six-week-old plants of each genotype were syringe-infiltrated with a mock solution (10 mM MgCl2) or with a X. gardneri strain 153 suspension (OD600=0.25). Samples were harvested six hours after infiltration.