Comparative gene expression in adult SVZ-neurospheres differentiated under standard conditions and upon manipulations of Pax6 (OE) and/or Pbx1 (KD/OE)

We used microarrays to detail the global programme of gene expression underlying neuronal differentiation of SVZ-neurospheres when manipulating Pax6 expression in the presence or absence of Pbx1 Freshly isolated stem- and progenitor cells from the SVZ of adult mice were subjected to four treatment regimes: i) infection with pCLIG, GFP-expressing retroviral vector viruses (vector ctrl), ii) infection with pCLIG expressing Pbx1 (Pbx1 OE), iii) transfection with non-targeting siRNAs followed by infection with pCLIG expressing Pax6 (Pax6 OE) and iv) transfection with siRNAs targeting Pbx1 followed by infection with pCLIG expressing Pax6 (Pax6 OE / Pbx1 KD). 48 hours later, the cells were differentiated for 10 hours on laminin-coated dishes by removal of EGF and FGF2 from the culture medium.