Omics-Lethal Human Viruses, Ebola Experiment EHUVEC001

The purpose of this experiment was to evaluate the human host response to Zaire Ebola wild-type virus and mutant virus infection in VP30 expression background. Samples were obtained from human umbilical cord endothelial cells (HUVEC) infected with wild-type Zaire Ebola virus in the ΔVP30 background (deltaVP30-WT) and mutant lacking the mucin domain (deltaVP30-deltamucin) encoding a glycoprotein lacking the mucin domain for mRNA transcriptome expression analysis. Secondary host-associated viral dataset downloads contain one or more statistically processed (normalization data transformation) quantitative dataset collections resulting in qualitative expression analyses of primary host-pathogen experimental study designs. Transcriptomics dataset downloads have a direct relationship to a primary sample submission corresponding to a specific Ebola virus infection.本实验旨在评估人宿主对扎伊尔埃博拉野生型病毒及VP30表达背景下突变病毒的感染应答。实验样本取自：经ΔVP30背景感染野生型扎伊尔埃博拉病毒（deltaVP30-WT）的人脐静脉内皮细胞（human umbilical cord endothelial cells, HUVEC），以及编码缺失黏蛋白结构域（mucin domain）糖蛋白的ΔVP30-Δ黏蛋白突变株（deltaVP30-deltamucin）感染的上述细胞，所有样本均用于mRNA转录组表达分析。二级宿主相关病毒数据集下载包含经统计学处理（归一化、数据转换）的定量数据集合集，可用于开展原代宿主-病原体实验研究设计的定性表达分析。转录组数据集下载与对应特定埃博拉病毒感染的原代样本提交存在直接关联。