The myomiR, miR-133, orchestrates myotome formation and embryonic myogenesis via the concerted regulation of hedgehog-pathway components

Cell fate decisions in developing embryos are tightly regulated and novel mechanistic insights may lead to improved methods to derive specific cell types, including muscle cells, for therapeutic purposes. The myomiR, miR-133, is pivotal for specification of the myogenic lineage; however, the detailed mechanisms by which it exerts its effects remain to be elucidated. Here, we report RNA sequencing datasets of chick embryo somites injected with antagomiR-133a or antagomiR-scrambled (control). The six most posterior somites from Hamburger and Hamilton (HH) stage 14 chick embryos were injected with fluorescein (FITC) labelled antagomiRs for microRNA-133a (1 uM) or scrambled-control (1 uM). Ten sets of six somites were harvested, pooled and RNA-seq was performed on each sample (three samples with antagomiR-133a and three samples with antagomir-scrambled control).