Novel hydrolase factors in septal PG splitting.

(A) Alleviating genetic interactions between annotated PG hydrolases and YceG/YebA. (B) Protein architecture (amino acid length indicated); domain structure of YceG unknown. CC, coiled coil; TM, transmembrane helix; SP, signal peptide. (C) Electron microscopy showing impaired membrane invagination and uncleaved PG septa (arrows) in E. coli mutants relative to wild-type cells; scale-bar 500 nm. (D) Cell division defects (arrows), phenotypic complementation (plasmid-based wild-type gene copy) or transgenic rescue. Strains stained with DAPI were visualized using a high content microscopy with differential interference contrast (DIC) and fluorescence optics; scale-bar 2 µm. (E) Differential strain growth with or without ampicillin (AMP, 5 µg/ml). (F) Alleviating double mutant interaction in liquid rich medium at 32°C over 24 h.