Transcriptome data of compatible soybean host inoculated with Sinorhizobium fredii with variants of NopP and NopI

Nodulation Outer Protein P (NopP) and NopI of the type 3 secretion system (T3SS) of the rhizobium determine host specificity. Sinorhizobium fredii CCBAU25509 (R2) and CCBAU45436 (R4) have different NopP and NopI variants, affecting their respective symbiotic compatibilities with the cultivated soybean C08 and the wild soybean W05. Swapping the NopP variants between R2 and R4 has been shown to switch their compatibility with C08 with the rj2/Rfg1 genotype. To understand the effects of Nops on host compatibility, analyses on the transcriptomic data of W05 roots and nodules inoculated with S. fredii strains containing Nop variants uncovered many differentially expressed genes related to nodulation and nodule functions, providing important information on the effects of Nops on hosts and nodules. To investigate the function of Nops in the regulationg of soybean inocualtion and nitrogen fixation, we swapped the NopP and NopI between Sinorhizobium fredii CCBAU25509 and CCBAU45436 and used these strains to infect soybean W05.