Respiratory Syncytial virus (RSV) directly binds host microRNAs to de-regulate host gene targets [RNA-seq miR-27 over-expression]

To validate miR-27 mRNA targets, we generated miR-27 overexpressing cell lines using the shMIMIC lentiviral transduction system (Dharmacon) In addition, we transfected miR-27 miRNA mimics and inhibitors to overexpress and inhibit the miRNA and performed total RNAseq RNA-seq data of A549 cells, WT and miR-27 overexpressing (shMIMIC) lines, as well as mimic and inhibitor transfected cells