Effect of NAC on the metabolic changes induced by osteopontin-c on deadherent cells.

MCF-7 cells transfected with osteopontin-c or vector control were plated in poly-HEMA coated wells. 4 mM N-acetyl L-cysteine was added to one sample of MCF-7 OPNc cells. After 48 hours, the cells were harvested and lysed for analysis of the metabolite levels by NMR. Shown are comparison of MCF-7 OPNc (C) to MCF-7 vector (V), comparison of MCF-7 OPNc + NAC to MCF-7 vector, comparison of MCF-7 OPNc to MCF-7 OPNc + NAC.Effect of NAC on the metabolic changes induced by osteopontin-c on deadherent cells.