In vivo isolated Wnt1-Prim1 Primary, Residual, and Recurrent Tumor Cells

Residual disease represents a major obstacle to the successful treatment of breast cancer, however little is known about the biology of residual tumor cells in vivo. To identify genes differentially expressed in vivo in residual tumor cells compared to primary tumor cells or recurrent tumor cells, we used flow cytometry to isolate GFP-labeled tumor cells from primary, residual, or recurrent tumors grown orthotopically in nu/nu mice. WTA was used to amplify RNA from all tumor cells. Three timepoints (Primary tumor, residual disease, recurrent tumor) were taken with 3-4 replicates per timepoint.