GCN2 drives macrophage and MDSC function and immunosuppression in the tumor microenvironment [ChIP-Seq]

The purpose of our study was to examine the role of GCN2 in the function of immunosuppressive cells such as tumor associated macrophages and MDSCs. We show that a myeloid lineage specific deletion of GCN2 results in a shift towards a more proinflammatory phenotype of tumor associated macrophages and MDSCs, thereby promoting anti-tumor immunity. Overall design: We determined the expression pattern of tumor associated macrophages and MDSCs, impact of ATF4 and GCN2 silencing in M0, M1 and M2 polarized macrophages as well as ATF4 binding sites in M0 or M2 polarized bone marrow derived macrophages using RNA-Seq, scRNA-Seq and ChIP-Seq.