Hippo deficient cardiac fibroblasts differentiate into osteochondroprogenitors (snRNA-Seq)

We investigated how Hippo deficient CFs alter their cell state and interact with immune cells during cardiac fibrosis. To study the role of the Hippo pathway in CFs, we conditionally knocked out Lats1/2 specifically in the CFs of mouse hearts (Lats1/2-CF) and performed single-nucleus RNA sequencing (snRNA-seq) to profile the cellular landscape of the left and right atria. Overall design: Single nuclei RNA sequencing were performed to examine left and right atrial cells, with or without Lats1 and Lats2 conditional knockout in the fibroblasts. All mice were adult with age of 10-12 weeks