16S rRNA V4 sequence metadata from surface swabs from Apostichopus californicus-associated flavivirus experiment under suboxic conditions and organic matter amendment

Microbial activities at the animal-water interface are hypothesized to influence viral replication and possibly contribute to pathology of echinoderm wasting diseases due to hypoxic stress. We assessed the impacts of enhanced microbial production and suboxic stress on Apostichopus californicus (urn:lsid:marinespecies.org:taxname:529363) associated flavivirus (PcaFV) load in a mesocosm experiment. This dataset contains 16S rRNA V4 amplicon sequencing metadata for this experiment. Organic matter amendment and suboxic stress resulted in lower PcaFV load, which also correlated negatively with animal mass loss and microbial activity at the animal-water interface. These data suggest that PcaFV replication and persistence was best supported in healthier specimens. Our results do not support the hypothesis that suboxic stress or microbial activity promote PcaFV replication, but rather that PcaFV appears to be a neutral or beneficial symbiont of Apostichopus californicus.学界曾提出假说：动物-水界面的微生物活动会影响病毒复制，并可能在低氧胁迫引发的棘皮动物消瘦症的病理进程中发挥作用。本研究通过中宇宙（mesocosm）实验，探究了微生物增殖增强与亚低氧胁迫对加州刺参（Apostichopus californicus, urn:lsid:marinespecies.org:taxname:529363）关联黄病毒（flavivirus，PcaFV）载量的影响。本数据集包含本次实验的16S rRNA V4区扩增子测序元数据。有机质添加与亚低氧胁迫可降低PcaFV载量，该载量同时与实验动物的体重损失及动物-水界面的微生物活动呈显著负相关。上述结果表明，PcaFV的复制与持续存在更易在健康状态更佳的个体中得到维持。本研究结果不支持“亚低氧胁迫或微生物活动可促进PcaFV复制”的初始假说，反而提示PcaFV更可能是加州刺参的中性或互利共生生物。