Biophysical properties of LmrCD-specific DARPins.

a)Derived from elution volume of main peak on Superdex 200 10/300 GL column (Figure S1).b)Determined by protein chip analysis (Figure 7B).c)Values obtained by SRP analysis using a 1∶1 binding model (Figure 7C).d)Value obtained by SPR analysis using binding equilibrium data (Figure 7D).e)Separation of the DARPin-LmrCD complex from DARPin aggregates was not possible on SEC (Figure S1).