Confocal images of TCS::GFP in wt and ahp4 mutants

This contains data taken by Elina Chrysanthou of the TCS marker in ahp4 and wild-type Arabidopsis plants. The data set was generated to test if there was cytokinin response was altered in the phloem companion cells of the mutant. The findings are published in Chrysanthou et al., 2025. All plants have been treated with 300 mM mannitol for 24h. Data series used for analysis consist of xzy stacks of 20 images. The folders are based on the time the images were taken. The marker was introduced to the ahp4-1 mutant by crossing and segregating lines genotyped. The files with the homozygous mutants are marked mutant and the wt plants that segregate are marked WT. Images are saved in .lif format and can be opened using either Leica's confocal software or using the Bioformats plugin for imageJ.本数据集包含埃莉娜·克兰桑图（Elina Chrysanthou）针对ahp4突变体与野生型拟南芥植株获取的TCS标记（TCS marker）相关实验数据。本数据集的构建旨在检测该突变体的韧皮部伴胞中细胞分裂素响应是否发生改变。相关研究成果已发表于Chrysanthou等人2025年的学术论文中。所有受试植株均经300 mM甘露醇处理24小时。用于分析的数据集序列由20张图像构成的xzy图像堆叠（xzy stacks）组成。数据集文件夹按照图像拍摄的时间进行组织。该TCS标记通过杂交及经基因型鉴定的分离株系筛选，被导入至ahp4-1突变体中。携带纯合突变体的文件标注为"mutant"，经分离得到的野生型植株文件标注为"WT"。所有图像均以.lif格式存储，可通过徕卡共聚焦软件或ImageJ的Bioformats插件打开。