Long-read sequencing and comparative genome analyses of seven Emergomyces type-strains

Emergomyces is a genus (Ajellomycetaceae, Onygenales) of dimorphic fungal pathogens that cause severe, opportunistic infections around the world. We used long-read nanopore sequencing to generate de novo genome assemblies followed by comparative analyses for the type-strains of Emergomyces africanus, Emergomyces canadensis, Emergomyces crescens, Emergomyces europaeus, Emergomyces orientalis, Emergomyces pasteurianus, and Emergomyces soli. The average Emergomyces genome was found to be 32,965,087bp in size (range 28,687,700–35,863,369bp) with GC%-contents of 42.01–45.72%. Furthermore, we formally validate the species descriptions for Emergomyces crescens and Emergomyces soli.