Detection of RNAs binding to Vg1 RNP
收藏NIAID Data Ecosystem2026-03-11 收录
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资源简介：
We report the application of NGS for high-throughput profiling of transcripts assoicated with the ribonuleoprotein (RNP) formed on Vg1 mRNA in oocytes of Xenopus Laevis. Biriefly, Biotinylated-Vg1 RNA was microinjected into Xenopus oocyte to allow formation of RNP complex in vivo. Next, streptavidin beads were added to the oocyte extract to specificly pull down the biotin-Vg1 RNP. After extensive washes, biotin-Vg1 RNP were eluted off by incubating the beads with free biotin and the associted RNAs were extracted by phenol and precipitated by ethanol.For RNA sequencing strategy, a single RNA library was prepared and sequenced on an Illumina MiSeq nano 300 cycle reagent kit.
创建时间：
2020-07-24
