SARS-CoV-2 CD8+ T cell killing assays using replicating viruses and transgenic antigens. Mateyka et al.

Supplementary videos of xCELLigence based target antigen killing assay of the brightfield channel overlayered with the RFP channel (2 images/second, 72h total) can be found online. Uploaded are videos for competent A549-Nsp2 cells as well as of competent A549-Gaussia cells with the addition of SARS-CoV-2-specific, TCR-engineered CD8+ T cells (E:T ratio 3:1, S13 and S32):
Movie S1 comp. A549-Nsp2 TCR S13 E_T 3_1, related to section ‘Expected outcomes’; Movie S2 comp. A549-Nsp2 TCR S32 E_T 3_1, related to section ‘Expected outcomes’; Movie S3 comp. A549-Gaussia TCR S13 E_T 3_1, related to section ‘Expected outcomes’; Movie S4 comp. A549-Gaussia TCR S32 E_T 3_1, related to section ‘Expected outcomes’.