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RNAseq analysis of mouse CD4+ regulatory Tregs in WT and ∆Foxp3 mice isolated from spleen and lungs.

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE166860
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RNAseq analysis of mouse CD4+ regulatory Tregs in 21-day-old male WT and ∆Foxp3 mice isolated from spleen and lungs. Mouse CD4+ regulatory Tregs in 21-day-old male WT and ∆Foxp3 mice were isolated from spleen and lungs by flow cytometry, as DAPI–TCRβ+CD4+GFP+CD25+. 1,000 cells were double-sorted directly into lysis buffer for RNA-seq (Smart-seq2) library preparation following the standard ImmGen low-input protocol (www.immgen.org).
创建时间:
2021-05-25
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