Effect of Shavenbaby on gene expression in S2 cells [RNAseq_S2]

Shavenbaby (Svb) transcription factor is involved in the differenciation of epidermal cells, the homeostasis of intestinal stem cell. Svb is first synthetized as a long repressor protein called SvbRepressor (SvbREP). In presence of Pri peptides Svb Repressor is clived by the proteasoome into a shorter activator protein called Svb Activator (SvbACT). We want in this study determine the effect of the two Svb forms on gene expression and determine the Svb target genes. We performed RNAseq experiment in S2 cell lines which present Svb REP or SvbACT or S2 cell without Svb. Overall design: The experiment was performed in cells that either did not show Svb (Ctrl) or showed either the SvbACT or SvbREP form. 2 independent replicates were done for each condition. Cells were harvested and RNA extraction was made with Rneasy Kit (Qiagen). Banks of reads and sequencing were done by HiSeq 200 (Illumina) at BGI at single-end 50nt per replicate.