BBBC039

该图像集是 U2OS 细胞高通量化学筛选的一部分，其中包含 200 种生物活性化合物的示例。治疗效果最初是使用细胞绘画测定法（荧光显微镜）成像的。该数据集仅包括每个化合物单个视野的 DNA 通道。这些图像呈现出各种核表型，代表高通量化学扰动。该数据集的主要用途是研究能够以准确方式分离单个核实例的分割算法，而不管它们的形状和细胞密度如何。该集合有大约 23,000 个手动注释的单核，以建立用于分割评估的地面实况集合。该数据集共有 200 个核视野，使用 Hoechst 染色通过荧光显微镜捕获。这些图像是较大的 BBBC022 化学屏幕的样本。图像存储为 16 位 520x696 像素的 TIFF 文件。

This image set is part of a high-throughput chemical screening campaign for U2OS cells, containing examples of 200 bioactive compounds. Treatment effects were initially imaged via the Cell Painting assay using fluorescence microscopy. This dataset only includes the DNA channel of a single field of view for each compound. These images display diverse nuclear phenotypes representing high-throughput chemical perturbations. The primary purpose of this dataset is to investigate segmentation algorithms that can accurately isolate individual nuclear instances regardless of their shape and cellular density. The collection contains approximately 23,000 manually annotated single nuclei to establish a ground truth set for segmentation evaluation. A total of 200 nuclear fields of view are included in this dataset, captured via fluorescence microscopy with Hoechst staining. These images are samples from the larger BBBC022 chemical screen, and are stored as 16-bit 520x696 pixel TIFF files.

搜集汇总

背景与挑战

背景概述

BBBC039是一个包含200种生物活性化合物荧光显微镜图像的细胞核分割数据集，共有约23,000个手动注释的单核实例，图像格式为16位520x696像素的TIFF文件，适用于核分割算法的研究。