RNA-seq analysis of pro-B cells from RAG, double RAG/Emu, double RAG/3'RR and double RAG/KOKI deficient mice.

By their impact on nuclear organisation, enhancers are master regulators of cell fate. We thus developed double Emu-RAG-deficient, 3’RR-RAG-deficient mice and KOKI-RAG-deficient mice to investigate a potential transcriptional cross-talk between Emu and 3'RR enhancers at the pro-B cell stage. Femoral pro-B cells were recovered with the EasySepTM mouse B-cell isolation Kit (STEMCELL Technologies, France). It is designed to isolate B cells from single-cell suspensions by negative selection. Pro-B cells were obtained from 10 RAG-deficient, 10 double 3’RR-RAG-deficient mice, 10 double KOKI-RAG deficient and 10 double Emu-RAG-deficient mice. Two pooled RNA (with five samples) were obtained for each genotype.