Whole-Slide Immunofluorescence Images of Human Placental and Umbilical Cord Tissues Demonstrating HER3 Expression and Cellular Architecture

Here we present our dataset of high-resolution whole-slide immunofluorescence images prepared for the research paper “A Dual Role for the Placenta and Human Amniotic Epithelial Cells in HER3-Targeted Nanotherapy.” The images in this dataset show the spatial distribution of epithelial cell marker (Pan-cytokeratin) and HER3 (ERBB3 receptor) in different sections of human placenta tissues, including the amniotic membrane and the umbilical cord tissues and their associated cell structures. These images were prepared to provide a resource for quantitative and qualitative analysis of HER3 localization in the human placenta tissues and for evaluating the interaction of HER3-targeting nanobioparticles (HPK2.0 and HerLLAA) with epithelial cell systems in human placenta tissues. The images in this dataset were generated from formalin-fixed paraffin-embedded (FFPE) sections of human placenta tissues that were processed by multiplex immunofluorescence and counterstaining delineating the following markers: DAPI: nuclear staining (blue) Pan-cytokeratin (pan-CK): epithelial cell marker (green) Cell surface HER3/ERBB3 (red) The images in this dataset represent the following anatomical sections of the human placenta tissues: Reflected amniotic membrane : The region of the human amniotic sac that lines the uterine wall, separate from the placental and umbilical cord regions. Placental amniotic membrane : The region of the human amniotic sac that lines the placental regions. Vascular structures of the umbilical cord (endothelium and vessel wall) from fetal side and maternal side of umbilical cord. Wharton’s jelly from fetal side and maternal side of umbilical cord. The scale bar represents 1000 µm. Human placental samples were obtained with informed consent and under approval of the Cedars-Sinai Institutional Review Board (IRB Approval ID: Pro00048705). All samples were de-identified prior to analysis.