Optimized reporters for multiplexed detection of transcription factor activity

Here, we use an MPRA to systematically optimize transcriptional reporters for 86 TFs and evaluate the specificity of all reporters across a wide array of TF perturbation conditions. We identified critical TF reporter design features and obtained highly sensitive and specific reporters for 60 TFs, many of which outperform available reporters. The resulting collection of prime TF reporters can be used to uncover TF regulatory networks and to illuminate signaling pathways.