Detection of individual fluorescently labeled reovirions in living cells.
收藏PubMed Central2026-05-16 收录
下载链接：
https://pmc.ncbi.nlm.nih.gov/articles/PMC54580/
下载链接
链接失效反馈官方服务：
资源简介：
Reovirus serotype 1 (Lang) can be conjugated with rhodamine B or fluorescein isothiocyanate in a way that preserves viral infectivity. We have used epifluorescence microscopy to detect individual virions bound to the surface of cells and to follow in real time the early stages of reovirus infection in living cells. Following uptake of the virus into endocytic vesicles, the movement of these vesicles can be observed readily. The vesicle movement is inhibited by nocodazole or colchicine, consistent with previous findings that the movement of intracellular vesicles is often microtubule-based. IMAGES:
提供机构：
National Academy of Sciences
