List of mutant strains and genes interrupted by the transposon.

Transposon insertion sites and locus tags are given in relation to the genome of the SCgG2 strain (NC_021352). Gene in operon are predicted from the transcriptome study of C. glutamicum ATCC13032 published by Pfeifer-sancar et al. [34]. ste hits correspond to genes identified by Lim et al. [13] from a screen based on an increased sensitivity to ethambutol of a library of mutants. Scores were assigned as described in the text: 1 or 2 for the immunological signal (see S1 Fig), 1 or 0 for the cell wall and secreted protein profiles (see S2 Fig) and 1 or 0 if the mutant exhibited at least one phenotypical or growth particularity (see text). TSS: transcription start sites, PO: primary operon, HP: hypothetical protein, HMP: hypothetical membrane protein, TMS: transmembrane segment, aa: amino acids. ND: not determined. (XLSX)