Next Generation Sequencing Facilitates Quantitative Analysis of Health donors and SLE patients'' PBMC Transcriptomes

Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare SLE patients to Health donors PBMCs-derived transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to discovery the potential pathway which can be used as a therapeutic target. Overall design: Methods: Peripheral blood from Health donors and SLE patients with high or low peli1 expression was collected,PBMCs was processed to RNA-sequencing.