Single-Cell RNA Sequencing unveils dynamic transcriptional profiles during the process of donkey spermatogenesis and maturation

With the increasing demand for donkey production, there has been a growing focus on the breeding of donkeys. However, our current understanding of the mechanisms underlying spermatogenesis and maturation in donkeys during reproduction remains limited.In this study, we constructed a single-cell RNA dataset to study the single-cell landscape of donkey spermatogenesis and maturation. This method allows us to analyze the cell composition in testicular and epididymal tissue, providing insights into the changes that occur during donkey spermatogenesis and maturation. In addition, different gene expression signatures associated with various spermatogenic cell types were found Testis and epididymis tissues of 3 sexually mature male donkeys were collected for single cell sequencing to explore the mechanism of spermatogenesis and maturation