Genome-wide maps of hnRNP UL1 binding on DNA during the inflammatory process.

We performed ChIP-seq with antibody targeting hnRNP UL1 at 0, 4h and 10h following LPS stimulation in RAW 264.7 cells. NGS and data analysis were done by Novogen China Co. Ltd. We found that hnRNP UL1 binds to chromatin DNA broadly and dynamically during inflammatory response. hnRNP UL1 binds to genes' promoter mainly. And NF-κB binding sites (κB sites) were significantly enriched in the motifs bound by hnRNP UL1. RAW 264.7 cells were stimulated by LPS for 0, 4h and 10h. Then protein-DNA complexes were sellectively immunoprecipitated using hnRNP UL1 antibody. Purified input DNA fragments and ChIP-derived DNA fragments were undergone NGS.