Microsatellite primers for the Pacific Northwest conifer, Callitropsis nootkatensis (Cupressaceae)

Premise of the study: Microsatellite primers were developed for Nootka cypress (Callitropsis nootkatensis) to provide quantitative measures for gene conservation that can assist in guiding management decisions for a species experiencing climate-induced decline. Methods and Results: Using multiplexed massively parallel sequencing, we identified 136,785 microsatellite-containing sequences from 489,625 paired-end 80 bp reads. After stringent filtering, we selected 144 primer pairs and screened variation at these loci in five populations of C. nootkatensis. Loci show between 3 and 36 dinucleotide repeats per locus, with an average of thirteen. Screening of these markers in the Pacific Northwest relative Chamaecyparis lawsoniana demonstrated no marker transferability. This finding highlights the narrow taxonomic utility of microsatellite markers in Callitropsis. Conclusions: These microsatellites show high polymorphism and can be used for routine screening of natural variation in Callitropsi...研究背景：本研究针对努特卡扁柏（Callitropsis nootkatensis）开发微卫星引物（microsatellite primers），旨在为该受气候诱导出现种群衰退的物种提供基因保护的量化评估手段，辅助制定科学管理决策。 方法与结果：本研究采用多重大规模并行测序（multiplexed massively parallel sequencing）技术，从489625条80bp双端测序读段（paired-end 80 bp reads）中筛选得到136785条含微卫星序列的片段。经严格过滤后，共选取144对引物，并在5个努特卡扁柏种群中检测了这些基因座的遗传变异情况。各基因座的二核苷酸重复次数介于3至36次之间，平均重复次数为13次。将上述标记在太平洋西北地区（Pacific Northwest）近缘物种美国扁柏（Chamaecyparis lawsoniana）中进行扩增验证，未检测到引物可转移性。该结果表明，微卫星标记在Callitropsis属内的分类学适用范围较为狭窄。 结论：本研究开发的微卫星标记具有较高的多态性，可用于常规筛查Callitropsi...自然群体的遗传变异。