Single-Cell RNA Sequencing of 7 PDA Cell Lines

We performed Single-Cell RNA Sequencing of 7 PDA Cell Lines to characterize and quantify PDA subtypes populations Overall design: Seven PDA cell lines (CAPAN1, HPAFII, HS766T, KP4, MIAPACA2, PANC0403, PATU8988S) were cultured and multiplexed together in order to generate baseline Single-Cell RNA-Sequencing from each one of them. To assess whether the confluence state of cell line would affect its protein activity profiles and, consequently, its cell state identification, the KP4 cell line was profiled at high-confluence states (KP4-HC) and low-confluence (KP4-LC).