Gene expression profile at single cell level of bone marrow cells in 5TGM1 multiple myeloma mouse model of NEK2 deficiency

To determine how the lack of NEK2 in the tumor microenvironmental cells contributes to tumor inhibition and immune regulation, we used single cell RNA sequencing (scRNA-seq) to analyze the diversity of immune cells in the bone marrow of 5TGM1 MM mouse model with and without NEK2 deficiency. Nek2 knockout mice had been backcrossed with C57BL/KaLwRij mice for 10 generations to allow for 5TGM1 MM cell growth. One million 5TGM1 MM cells were injected into the Nek2 wild-type and knockout mice via tail vein. Bone marrow cells were harvested on day 30 post-5TGM1 cell injection and processed for scRNA-seq analysis.