Toxoplasma candidate apical proteins identified by hyperLOPIT and BioID methods.

Footnotes: aKnown localization defined as “apex” when low-resolution imaging only has identified a punctum at the apex of the cell. AA, apical annuli; APR, apical polar ring; CCR, conoid canopy ring; ICMT, intraconoidal microtubules; PM, plasma membrane: bLOPIT assignment strength: ● (black), Protein assigned to one of the 2 apical clusters at >99% probability in (before “/”) the analysis of concatenated 3 or 2 datasets where the protein was full quantified or (after “/”) the analysis of individual hyperLOPIT datasets; ● (grey), as for above except apical assignment probability is below the 99% threshold; ● (yellow), assignment to a cluster other than apical in 1 noncatenated hyperLOPIT datasets. For BioID proteomic experiments, the detection of a protein by a given bait is indicated by ●. For Hu’s and colleagues’ 2006 proteomic study, proteins enriched with conoid fractions by at least 2-fold (ToxoDB Release 49) are indicated by ●: cMutant phenotype fitness scores where more strongly negative scores indicate increasing detrimental competitive growth in in vitro culture conditions for T. gondii [40].* References for localization data: [31–34,36,39,46,49,62–64,66,68,69,71–80]; TS, this study. (XLSX)