de novo genome assemblies of black-tailed dusky antechinus (Antechinus arktos), silver-headed antechinus (Antechinus argentus), and black-tailed dasyure (Murexia melanurus)

de novo genome assemblies of the dasyurids black-tailed dusky antechinus (Antechinus arktos; assembly AA100A) and silver-headed antechinus (Antechinus argentus; assembly BD-17-5A) of Australia, and a dasyure from Papua New Guinea (Murexia melanurus; assembly 46020A).

Paired-end (100 bp) DNA libraries were sequenced by BGI (Hong Kong) using a BGISEQ-500 instrument to generate ~30× genome coverage. Raw data (mean 948,503,285±3,100,231 reads) was filtered using Flexbar v3.4.0 [1,2] (to remove adapters and low-quality reads) with default settings. Whole-genome sequencing reads were used to generate de novo assemblies using SGA v0.10.15 [3]. Resulting scaffolds were gap filled using ‘sga gapfill’ and error-corrected FASTQ reads. Please see https://github.com/sciseim/semelparity for associated scripts.

References
1    Roehr, J. T., Dieterich, C. & Reinert, K. Flexbar 3.0 - SIMD and multicore parallelization. Bioinformatics 33, 2941-2942, doi:10.1093/bioinformatics/btx330 (2017).
2    Dodt, M., Roehr, J. T., Ahmed, R. & Dieterich, C. FLEXBAR-Flexible Barcode and Adapter Processing for Next-Generation Sequencing Platforms. Biology (Basel) 1, 895-905, doi:10.3390/biology1030895 (2012).
3    Simpson, J. T. & Durbin, R. Efficient de novo assembly of large genomes using compressed data structures. Genome Res 22, 549-556, doi:10.1101/gr.126953.111 (2012).