Plant regeneration through direct somatic embryogenesis, antioxidant properties, and metabolite profiles of <i>Swertia corymbosa</i> (Griseb.) Wight <i>ex</i> C.B. Clarke

A protocol for induction of direct somatic embryogenesis and subsequent plant regeneration for the medicinally important and endangered plant of <i>Swertia corymbosa</i> has been developed for the first time. In the present study, <i>in vitro</i> derived leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) individually and in combination with cytokinins for its effectiveness to induce the differentiation of somatic embryos. The antioxidant activities of methanolic extracts from non-embryonic callus (NEC), pre-embryoid masses (PEM), somatic embryos at globular stage (SEG), somatic embryos at heart-shaped stage (SEHS), and cotyledonary embryos (SEC) of <i>S. corymbosa</i> were evaluated using three <i>in vitro</i> assays: scavenging of free radicals (DPPH and ABTS) and ferric reducing antioxidant test. In all cases, the methanolic extract from SEG demonstrated better antioxidant activity than those taken from other tested samples. Higher amounts of swertianin (<b>1</b>), methylswertianin (<b>2</b>), and 1,2- dihydroxy-6-methoxyxanthone-8-O-β-D-xylopyranosyl (<b>3</b>) were found in SEG stage followed by SEHS and PEM when compared to NEC and SEC.

本研究首次建立了兼具重要药用价值与濒危保护地位的伞房獐牙菜（Swertia corymbosa）直接体细胞胚胎发生及后续植株再生的实验规程。本研究以离体培养获得的叶片外植体为材料，分别以单独添加2,4-二氯苯氧乙酸（2,4-D），以及2,4-D与细胞分裂素类组合的Murashige和Skoog（MS）培养基进行培养，以验证其诱导体细胞胚胎分化的效果。此外，本研究选取伞房獐牙菜的非胚性愈伤组织（NEC）、前胚性细胞团（PEM）、球形期体细胞胚胎（SEG）、心形期体细胞胚胎（SEHS）及子叶形体细胞胚胎（SEC）的甲醇提取物，采用三种离体检测方法评价其抗氧化活性：自由基清除实验（DPPH与ABTS法）及铁还原抗氧化实验。所有检测结果均显示，球形期体细胞胚胎的甲醇提取物抗氧化活性优于其余所有供试样品。与非胚性愈伤组织和子叶形体细胞胚胎相比，球形期体细胞胚胎中獐牙菜素（swertianin，1）、甲基獐牙菜素（methylswertianin，2）及1,2-二羟基-6-甲氧基呫吨酮-8-O-β-D-吡喃木糖苷（1,2-dihydroxy-6-methoxyxanthone-8-O-β-D-xylopyranosyl，3）的含量更高，其后依次为心形期体细胞胚胎与前胚性细胞团。

搜集汇总

背景与挑战

背景概述

该数据集聚焦于药用濒危植物Swertia corymbosa，首次开发了其直接体细胞胚胎发生和植物再生协议，并系统评估了不同发育阶段样本的抗氧化活性和代谢物含量。研究发现，球形阶段体细胞胚胎（SEG）具有最佳的抗氧化活性，且含有较高水平的swertianin等关键代谢物，为植物再生和药用价值研究提供了重要数据支持。