Homing endonuclease I-PpoI-DNA complex:reaction at pH8.0 (Tris) with 500 uM Mg2+ for 10s

Homing endonuclease I-PpoI-DNA complex:reaction at pH8.0 (Tris) with 500 uM Mg2+ for 10s Descriptor: DNA (5'-D(*TP*TP*GP*AP*CP*TP*CP*TP*CP*TP*TP*AP*AP*GP*AP*GP*AP*GP*TP*CP*A)-3'), GLYCEROL, Intron-encoded endonuclease I-PpoI, ... Authors: Chang, C, Gao, Y. Deposit date: 2024-01-13 Release date: 2024-07-10 Last modified: 2025-01-29 Method: X-RAY DIFFRACTION (1.54 Å) Cite: Observing one-divalent-metal-ion-dependent and histidine-promoted His-Me family I-PpoI nuclease catalysis in crystallo. Elife, 13, 2024

归巢内切酶（homing endonuclease）I-PpoI-DNA复合物：于pH 8.0的Tris缓冲体系中，以500 μM镁离子（Mg²+）反应10秒。描述项：DNA（5'-D(*TP*TP*GP*AP*CP*TP*CP*TP*CP*TP*TP*AP*AP*GP*AP*GP*AP*GP*TP*CP*A)-3'）、甘油、内含子编码的归巢内切酶I-PpoI……作者：Chang, C、Gao, Y。沉积日期：2024-01-13；发布日期：2024-07-10；最后修改日期：2025-01-29。实验方法：X射线衍射（1.54 埃）。引用：《在晶体中观测到依赖单二价金属离子且受组氨酸促进的His-Me家族I-PpoI核酸酶催化反应》，eLife，13卷，2024年。