Analysis of differentially expressed genes in intrahepatic HBsAg accumulating mice. Analysis of differentially expressed genes in intrahepatic HBsAg accumulating mice

Search for distinct genes whose expression correlates with IFN⍺ mediated liver injury in HBsAg-Tg mice. IFN treatment induces liver injury from 8 hours post IFN treament, peaking at 24 hours, in HBs transgenic mice and not in the non-transgenic (control) mice Overall design: snap-frozen liver samples were obtained from groups of four HBsAg-Tg (Tg) and control mice (CTL) sacrificed prior to (0 hour) and 2, 4, 8 and 24 hours after treatment with 5MU/kg of IFN⍺. RNA was analysed from duplicate biological replicates . Groupings A-E correspond to the timepoints 0,2,4,8 and 24 hours.