Evaluation of immuno-reactive epitopes in the sera and cerebrospinal fluid of patients with post-treatment Lyme disease syndrome

While most patients fully recover after treatment for Lyme disease with recommended antibiotic regimens, some report non-specific symptoms after treatment. When these symptoms are unexplained by other conditions and persist for >6 months, this condition is called post-treatment Lyme disease symptoms or syndrome (PTLDS). The pathogenesis of PTLDS is unknown, and no specific diagnostic biomarkers have been identified. In this study, we used a high-density peptide array to examine antibody responses to >60 primary antigens of B. burgdorferi from a cohort of patients diagnosed with PTLDS and recovered patients with similar Lyme disease manifestations. Using matched serum and cerebrospinal fluid (CSF), we mapped the primary reactive B. burgdorferi epitopes associated with PTLDS. We found that VlsE had a greater antibody response within the PTLDS cohort than recovered patients. The reactivity to OspC-specific epitopes revealed a predominance of antibodies to OspC type K and A in the PTL..., , # Evaluation of immuno-reactive epitopes in the sera and cerebrospinal fluid of patients with post-treatment Lyme disease syndrome

Dataset DOI: [10.5061/dryad.wpzgmsc1f](10.5061/dryad.wpzgmsc1f)

## Description of the data and file structure

Relative fluorescent unit signals for 12-mer peptides originating from >60 antigens of Borrelia burgdorferi. The data includes IgM and IgG signal from sera and cerebrospinal fluid from 41 patients diagnosed with post-treatment Lyme disease. It also includes IgG signal data from 37 controls designated as patients with Lyme disease without a post-treatment Lyme disease diagnosis.

### Files and variables

#### File: PTLDS_SAMPLE_METADATA_IgG_IgM.xlsx

**Description:** Metadata file for IgG and IgM split between sheet 1 and sheet 2, respectively. Both sheets contain serum and CSF sample information. 

##### Variables

* Raw_Sample_ID: Sample ID that corresponds to columns in data files.
* Sample_LabID: ID given to each sample designating sample numbe..., Samples were collected under clinical protocols approved by the National Institutes of Health (NIH) institutional review board (ClinicalTrials.gov Identifier: NCT00028080 and NCT00001539), all methods were performed in accordance with the relevant guidelines and regulations, and written informed consent was obtained from all participants.

Samples were de-identified prior to analysis at Columbia University. Each sample at the NIH received a unique numerical identifier prior to shipment and analysis at Columbia University. No identifiable information could be obtained from the numerical identifier. ,