DMS-MaPseq raw sequencing data for HOTSCRAMBL RNA structure profiling

Raw sequencing data from dimethyl sulfate mutational profiling with sequencing (DMS-MaPseq) experiments used to probe the RNA secondary structure of HOTSCRAMBL and the rs17437411 variant. RNA was generated by in vitro transcription using a T7 promoter and refolded in sodium cacodylate buffer with MgCl₂ prior to chemical modification. RNA was treated with 3% DMS at 37 °C for 5 min, followed by quenching with β-mercaptoethanol. Modified RNA was reverse transcribed, PCR amplified, and sequencing libraries were prepared using the NEBNext UltraExpress DNA Library Prep Kit. Libraries were sequenced on an Illumina NextSeq 1000 platform (2 × 150 bp paired-end reads). Two biological replicates were generated for each HOTSCRAMBL variant, together with corresponding no-DMS control samples. Sequencing data were analyzed using SEISMIC (v24.3) to quantify DMS reactivity, cluster alternative RNA structures, and evaluate secondary structure models. DMS signals were normalized using Yeo–Johnson power transformation prior to structural analysis.