Revealing tumour cell heterogeneity in an Aqp5-APK mouse pyloric tumour model

The cell lineages present within the healthy gastric epithelium have been well delineated. However, tumour cell lineages within the stomach are less understood. To dissect the heterogeneity within pyloric tumours in the mouse stomach, we performed scRNAseq on an Aqp5-APK mouse pyloric tumour to resolve the diversity of cell populations present within these tumours. Overall design: An Aqp5-eGFP-ires-CreERT2; Apcfl/fl; Ptenfl/fl; KrasG12D/+ mouse was induced with two doses of 4mg tamoxifen and the resultant tumours harvested after 10 weeks. Tumours were dissociated to single cells and processed for scRNAseq.