Figure 6: Immunohistochemical analysis of immediate-early gene (IEG) recruitment after DSR testing.

Both Dom and Sub mice demonstrated markedly increased c-Jun (A: LSN, C: Cg) and EGR-1 (B: LSN, D: Cg) immunoreactivity following their respective social victory or defeat in the DSR test. IEG signal was markedly stronger among socially victorious Dom mice in the LSN (A: c-jun, B: EGR-1), while immunoreactivity in the Cg was more highly elevated among socially defeated Sub mice (C: c-jun, D: EGR-1). Data are presented as mean ±SEM, n=6 mice from each strain-treatment group; variables contributing to statistical significance by two-way ANOVA indicated as: DSR testing (+-p&lt;0.05, ++++-p&lt;0.0001), strain (####-p&lt;0.0001) and strain-DSR interaction (^^^-p&lt;0.001, ^^^^-p&lt;0.0001); Dom: Dominant, Sub: Submissive, LSN: lateral septal nucleus, Cg: cingulate gyrus.在DSR测试中经历对应社交获胜或失败后，优势（Dominant, Dom）小鼠与顺从（Submissive, Sub）小鼠的c-Jun及EGR-1免疫反应性均显著升高：其中c-Jun的免疫染色对应图A（外侧隔核，lateral septal nucleus, LSN）与图C（扣带回，cingulate gyrus, Cg），EGR-1的免疫染色对应图B（LSN）与图D（Cg）。社交获胜的Dom小鼠在LSN中的即刻早期基因（Immediate Early Gene, IEG）信号显著更强（c-Jun对应图A，EGR-1对应图B），而社交失败的Sub小鼠在Cg中的免疫反应性升高更为显著（c-Jun对应图C，EGR-1对应图D）。所有数据以平均值±标准误（mean ± standard error of the mean, SEM）呈现，每个品系-处理组的样本量为6只小鼠；双因素方差分析（two-way ANOVA）显示具有统计学显著性的变量包括：DSR测试（+-p<0.05，++++p<0.0001）、品系（####p<0.0001）以及品系与DSR测试的交互作用（^^^p<0.001，^^^^p<0.0001）；其中Dom指代优势（Dominant）小鼠，Sub指代顺从（Submissive）小鼠，LSN指代外侧隔核（lateral septal nucleus），Cg指代扣带回（cingulate gyrus）。