Individual vacuoles from untreated wildtype yeast cells at steady state. These vacuoles were classified as spherical. These images are supplementary data for my lab's PLoS ONE paper, which is lin
The ascorbate peroxidase APEX2 is commonly used to study the neighborhood of a protein of interest by proximity-dependent biotinylation. Here, we describe a protocol for sample processing compatible w
This dataset provides segmented images that correspond to the raw data in the "centrosomes" folder. Users can use these segmented images to run the SubcellularDistribution pipeline if they wan
Immunogold label specific for L1 antigen on DV-II. Monoclonal antibody to antigen L1 specifically labels DV-II and acidosomes but not lysosomes. Lysosomes are osmotically active so they tend to shrink