A comparative analysis of reactive Müller glia gene expression after light damage and microRNA-depleted Müller glia – focus on microRNAs [NanoString]. A comparative analysis of reactive Müller glia gene expression after light damage and microRNA-depleted Müller glia – focus on microRNAs [NanoString]

In order to identify the miRNAs in adult light damaged Muller glia of the the neural retina, we isolated the Müller glia from Rlbp-CreER: Stopf/f-tdTomato by means of fluorescent activated cell sorting and analyzed their miRNAs using NanoStrings Technologies®. We compared the data to that from and Dicer1-CKO Müller glia and wild type glia Müller glia (GSE103098). We found three miRNAs potentially regulating genes involved in stress response in both data sets. Overall design: Fluorescence activated cell sorting (FACS) was used to purify adult light damaged Müller glia (pooled from 44 retinas, > postnatal day 21), using an Müller glia specific cre-lox labeling strategy by crossing Rlbp1-CreER with tdTomato mice. The RNA was extracted from purified Müller glia (tdTomato+) and miRNA expression was analysed using NanoString and nCounter Technologies ®.