The content of C18:2-, C18:1-, C18:0- and C16:0-LPC in different life-cycle stages of <i>T. cruzi</i>.

aThe molar relative response factors (MRRF) of C10:0-LPC and LPC standards were used to calculate the amount of each LPC molecular species in Folch lower-phase fractions of T. cruzi. bThe number of parasites was determined before lipid extraction by counting live parasites in a hemocytometer. Values are means of three determinations. The standard deviation in all cases was <15%. cDetermined by multiplying the number of moles by the Avogadro's constant. dEstimated based on the parasite's length and diameter as determined by scanning electron microscopy, and assuming that each parasite is cylindrical, as previous described [103]. eObtained by dividing the number of LPC molecules per cell by the surface area of each parasite form. fObtained by dividing the amount of picomoles of LPC per 106 cells of each parasite form (column 1) by the values obtained for Epi. gNot analyzed due to absence or trace amounts of the compound.